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mirrorball®: high sensitivity cytometry for cell based assays

     
NO.: mirrorball Specifications: 
brand:  Storage: 常规储存

mirrorball®: high sensitivity cytometry for cell based assaysProduct description

For cell based assays, TTP Labtech’s mirrorball® high sensitivity cytometer represents one of the most versatile instruments available today.

mirrorball’s flexibility allows direct transfer of cell based assays that normally require ABI 8200 (FMAT®), high content imaging or flow cytometry. It is the first system in its class to offer simultaneous scanning with multiple lasers (405, 488 and 640 nm) and offers a unique set of enabling features:

■ Homogenous no-wash-assays: - eliminating wash procedures yet retaining high sensitivity. mirrorball is capable of performing cell based assays compatible with both adherant a non-adherent cell lines.

■ High-speed multiplexing: - for identification of multiple fluorophores and analysis of heterogeneous populations in bead and cell based assays.

■ High content imaging: - with whole well scanning, mirrorball requires fewer cells to achieve statistically significant results.

■ Flow cytometry in a microplate: - with high sensitivity, mirroball’s offers an alternative to flow cytometry for the detection of low affinity antibodies and proteins found in low abundance.


mirrorball®: high sensitivity cytometry for cell based assaysapplication area

The versatility of mirrorball® permits both cell- and bead-based, fluorescent assays. It is ideally suited to mix-and-read assays suitable for antibody screening. In addition, the simultaneous, triple laser excitation and multicolour fluorescence detection capability of mirrorball addresses a broad range of high content cellular screening applications.

Cell-based assays:
■    screening for whole antibodies and antibody fragments
■    phage library screening
■    cell surface receptor expression
■    cytotoxicity
■    proliferation
■    cell cycle analysis

Bead-based applications:
■    antibody screening for soluble antigens
■    quantitation of soluble proteins, e.g. chemokines   


mirrorball®: high sensitivity cytometry for cell based assaystechnical parameter

Detection technology

Laser scanning fluorescence cytometry

Laser excitation

Up to 3 solid state laser in a single instrument (choice includes 405、488、640nm)

Multi-color detection

Up to 5 colors simultaneously using photomultiplier tubes

Laser scatter detection

Single channel(beads only)

Scan area

Whole well

Sample format

96, 384 and 1536 SBS-format microplates

Throughput

Typically 10-15 minutes regardless of plate type

File export

CSV、FCS and open TIFF(8-&16-bit)

Software

Cellista software compatible with Microsoft Windows 7(Professional)

Configurations

Stand-alone, self-maintained workstation or fully integrated

Laser safety

Class 1 laser product

Dimensions

645mm x 513mm x 350mm (w x d x h)

Net weight

Approx 60kg (132lbs)

Services

110/230V single phase 47/63Hz 800W


mirrorball®: high sensitivity cytometry for cell based assaysRemarks and notes

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